Fall 2013 H7N9 Human Genetic Diversity

Sequences discussed in this analysis are variously stored publicly at GenBank and at GISAID. We gratefully acknowledge the authors, originating and submitting laboratories of the sequences from GenBank and from GISAID’s EpiFlu™ Database on which this research is based. A GISAID-generated list is detailed in a linked Excel for completeness in citation.

Publish Date : 2013-12-09
Last Update : 2013-12-09

The GeneWurx Comparative targeting five segments from the Antigens & the Polymerase Complex demonstrates the genetic diversity of the four most recently deposited human eH7N9 sequences covering three cases.  The genetic record shows that at least three distinct constellations have circulated during late summer and fall of 2013.

The active acquisition of segment & sub-segment genetic material from avian reservoirs, circulating human seasonal reservoirs (pH1N1 & sH3N2) and human / zoonotic serotypes is certainly a point of interest. This charted high temporal concentration of variance suggests that the Emergent H7N9 reservoir is in full Host Switching mode and is seeking Gain of Function with Antigenic Novelty.

Human H7N9 Segment Comparative
Guangdong Huizhou 51F 2013_08_10 (GD) Seg Sero Note  HA   H7N9   Novel   NA   H7N9   Base   PB2   H9N2   Assort   PB1   H9N2   Assort   PA   H7N9   Novel	Zhejiang Shaoxing 35M 2013_10_14 (ZC) Seg Sero Note  HA   H7N9   Novel   NA   H7N9   Novel   PB2   H7N9   AvBase   PB1   H7N9   Novel   PA   H7N9   =ZU	Zhejiang Shaoxing 35M 2013_10_14 (ZU) Seg Sero Note  HA   H7N9   Novel   NA   H7N9   Novel   PB2   H7N9   Novel   PB1   H7N9   Novel   PA   H7N9   =ZC	Hong Kong 36F 2013_11_30 (HK) Seg Sero Note  HA   H7N9   ~,!=GD   NA   H7N9   Novel   PB2   H9N2   Assort ~,!=GD   PB1   H9N2   Assort !~GD   PA   H9N2   Assort
Deposit Report	Deposit Report	Deposit Report	Deposit Report

Please visit GeneWurx.com for insight into the latest published studies.

Publication Copyright 2007-2013 GeneWurx.com, All Rights Reserved.

Shaoxing, Zhejiang Fall 2013 Human H7N9 Displays Varied Genetics from Two Laboratories

Sequences discussed in this analysis are variously stored publicly at GenBank and at GISAID. We gratefully acknowledge the authors, originating and submitting laboratories of the sequences from GenBank and from GISAID’s EpiFlu™ Database on which this research is based. A GISAID-generated list is detailed in a linked Excel for completeness in citation.

Publish Date : 2013-12-08
Last Update : 2014-02-11

On 2013-10-16, the Zhejiang CDC rapidly released a full set of sequences at GISAID from a  Emergent H7N9 human case sampled from a 35 year old male who resided in Shaoxing, Zhejiang. The sample was taken on October 14, 2013 during hospitalisation. The patient showed no underlying diseases, nor reported any direct contact with poultry. This Shaoxing, Zhejiang patient is indicated as Case #137 on the 2013 H7N9 Human Case List maintained by the crowd-sourced FluTrackers.com.

On 2013-10-18, Zhejiang University also rapidly released a full set of sequences at GISAID from the same human case with the same sample collection date.  One week later on 2013-10-25, their sequence was updated and renamed.

Neither organisation indicated a passage history for their respective sequences, but the Zhejiang University sequences manifested additional polymorphisms.

The antigens are identical, but the Polymerase Complex varies between the two laboratories.  A novel HA 222P was confirmed by the Zhejiang University deposit. Although the Polymerase vary, these human sequences do share homology at the PB2 627E, generally an avian value. The Polymerase Acidic segments are identical, but the Zhejiang University PB2 and PB1 segments demonstrate polymorphisms not found on the Zhejiang CDC segments.

The Zhejiang CDC issued a press release indicating in translation at Crawford Kilian's H5N1 site that "no significant variation" was found on this case. On our detailed examination of the sequence, variation is in evidence. Perhaps novelty on Antigens is not considered "significant"  by the expert official delivering the public relations missive. A GeneWurx Comparative of the Antigens and Polymerase Complex against the recent human sequences demonstrates what we consider to be significant variation within circulating Emergent H7N9.

The sequences from this October 2013 Zhejiang case do not resemble the genetics from the two current human sequences that border on the calendar, GuangdongHuizhou1 at 2013_08_10 & HongKong5942 at 2013_11_30.

HA Polymorphisms

. . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/22/2013

. . . . . . . . Hemagglutinin Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s

. . . . . . . . Novel Hemagglutinin, 1 Case Over 2 Sequences
. . . . . . . . GISAID HA EPI477410

. . . . . . . . GISAID Isolate EPI_ISL_148728

. . . . . . . . 107 Polymorphisms (18 Amino and 89 Silent)

. . . . . . . . 11I [#4I],

. . . . . . . . syn74L (CTa) [syn60L (CTa)] [H7N9 Novel],

. . . . . . . . . . . . . . . . . .[pH1N1 syn60I ATa 3^rd Base Donor,

. . . . . . . . . . . . . . . . . . . . . . . ReEmergent 2013,

. . . . . . . . . . . . . . . . . . . . . . . Japan, Corsica 2013-January,

. . . . . . . . . . . . . . . . . . . . . . . Asia Extensive 2010, 2011],

. . . . . . . . . . . . . . . . . .[H5N1 syn60V GTA 3^rd Base Donor,

. . . . . . . . . . . . . . . . . . . . . . . Canada Alberta Human 2013], 

. . . . . . . . syn108G (GGa) [syn96G (GGa)] [Emergent H7N9 Novel],

. . . . . . . . . . . . . . . . . . . [H7N9 Rare],

. . . . . . . . . . . . . . . . . . . [pH1N1 wild type],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Human

. . . . . . . . . . . . . . . . . . . . . . . . . . including Karo cluster

. . . . . . . . . . . . . . . . . . . . . . . . . . . . and Recent Fatality,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IndonesiaNIHRD13157_f
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30mM 2013_06_18],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Feline 2006],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Passerine 2010],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Commercial Poultry 2006-2010],

. . . . . . . . . . . . . . . . . . . [H9N2 Korea Commercial Poultry Rare 2006],

. . . . . . . . . . . . . . . . . . . [H9N2 Rare migratory birds 2008-2009],

. . . . . . . . . . . . . . . . . . . [H7N7 Italy Commercial Poultry

. . . . . . . . . . . . . . . . . . . . . . . . . . including Recent

. . . . . . . . . . . . . . . . . . . . . . . . . . . . ckItaly13VIR452711_2013_08_14],

. . . . . . . . . . . . . . . . . . . [H7N7 Massachusetts Sea Mammal 1980],

. . . . . . . . . . . . . . . . . . . [H7N7 Worldwide Equine wt 1956-1977],

. . . . . . . . . . . . . . . . . . . [H7N7 United States migratory birds 2006-2011],

. . . . . . . . . . . . . . . . . . . [H7N7 Egypt Passerine 2004],

. . . . . . . . . . . . . . . . . . . [H6N1 wild type],

. . . . . . . . 130A [118A],

. . . . . . . . 183S [174S],

. . . . . . . . 188V [179V],

. . . . . . . . 195V [186V],

. . . . . . . . 198A [189A],

. . . . . . . . 211V [202V],

. . . . . . . . 217N [208N],

. . . . . . . . 231P [222P] [H7N9 Novel],

. . . . . . . . . . . . . . . . .[H7N9 LookAside_u],

. . . . . . . . . . . . . . . . .[pH1N1 LookAside_u],

. . . . . . . . . . . . . . . . .[H9N2 Hong Kong Avian Rare 2012],

. . . . . . . . . . . . . . . . .[H7N7 Avian Rare 1975-1976 ],

. . . . . . . . . . . . . . . . .[H6N1 Avian Singular 2002],

. . . . . . . . . . . . . . . . .[H6N2, N3, N5, N6, N7, N8],

. . . . . . . . 235L [226L],

. . . . . . . . 285N [277N],

. . . . . . . . 307D [299D],

. . . . . . . . 321R [313R],

. . . . . . . . 410N [401N],

. . . . . . . . 427I [418I],

. . . . . . . . 455D [446D],

. . . . . . . . 462K [453K],

. . . . . . . . syn517L (tTA) [syn508L (tTA)] [Emergent H7N9 Novel],

. . . . . . . . . . . . . . . . . . [H7N9 North America Avian wild type (tTg)],

. . . . . . . . . . . . . . . . . . [pH1N1 (tTg)],

. . . . . . . . . . . . . . . . . . [H1N1v Human 2012-2013 (tTg)

. . . . . . . . . . . . . . . . . . . . . . . . . including Arkansas14_2013_09_06],

. . . . . . . . . . . . . . . . . . [H1N1 Seasonal 1951],

. . . . . . . . . . . . . . . . . . [H5N1 Human and Avian 2013 (tTg)

. . . . . . . . . . . . . . . . . . . . . . . . . including Recent Fatality,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . IndonesiaNIHRD13157_f
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30mM 2013_06_18],

. . . . . . . . . . . . . . . . . . [H3N2],

. . . . . . . . . . . . . . . . . . [H7N7],

. . . . . . . . . . . . . . . . . . [H6N1 Rare Count < 10],

. . . . . . . . 541V [533V])

. . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/DTID-ZJU10/2013

. . . . . . . . renamed after 2013-10-18 Deposit from

. . . . . . . . . . . . A/Zhejiang/DTID-ZJU05/2013

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU05_35M_2013_10_14_s

. . . . . . . . Hemagglutinin Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s

. . . . . . . . Novel Hemagglutinin, 1 Case Over 2 Sequences
. . . . . . . . GISAID HA EPI477450

. . . . . . . . GISAID Isolate EPI_ISL_148744

. . . . . . . . 107 Polymorphisms (18 Amino and 89 Silent)

. . . . . . . . HA Truncated before aa3 [HA Truncated before aa#12],

. . . . . . . . 11I [#4I],

. . . . . . . . syn74L (CTa) [syn60L (CTa)] [H7N9 Novel],

. . . . . . . . . . . . . . . . . .[pH1N1 syn60I ATa 3^rd Base Donor,

. . . . . . . . . . . . . . . . . . . . . . . ReEmergent 2013,

. . . . . . . . . . . . . . . . . . . . . . . Japan, Corsica 2013-January,

. . . . . . . . . . . . . . . . . . . . . . . Asia Extensive 2010, 2011],

. . . . . . . . . . . . . . . . . .[H5N1 syn60V GTA 3^rd Base Donor,

. . . . . . . . . . . . . . . . . . . . . . . Canada Alberta Human 2013], 

. . . . . . . . syn108G (GGa) [syn96G (GGa)] [Emergent H7N9 Novel],

. . . . . . . . . . . . . . . . . . . [H7N9 Rare],

. . . . . . . . . . . . . . . . . . . [pH1N1 wild type],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Human

. . . . . . . . . . . . . . . . . . . . . . . . . . including Karo cluster

. . . . . . . . . . . . . . . . . . . . . . . . . . . . and Recent Fatality,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . IndonesiaNIHRD13157_f
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30mM 2013_06_18],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Feline 2006],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Passerine 2010],

. . . . . . . . . . . . . . . . . . . [H5N1 Indonesia Commercial Poultry 2006-2010],

. . . . . . . . . . . . . . . . . . . [H9N2 Korea Commercial Poultry Rare 2006],

. . . . . . . . . . . . . . . . . . . [H9N2 Rare migratory birds 2008-2009],

. . . . . . . . . . . . . . . . . . . [H7N7 Italy Commercial Poultry

. . . . . . . . . . . . . . . . . . . . . . . . . . including Recent

. . . . . . . . . . . . . . . . . . . . . . . . . . . . ckItaly13VIR452711_2013_08_14],

. . . . . . . . . . . . . . . . . . . [H7N7 Massachusetts Sea Mammal 1980],

. . . . . . . . . . . . . . . . . . . [H7N7 Worldwide Equine wt 1956-1977],

. . . . . . . . . . . . . . . . . . . [H7N7 United States migratory birds 2006-2011],

. . . . . . . . . . . . . . . . . . . [H7N7 Egypt Passerine 2004],

. . . . . . . . . . . . . . . . . . . [H6N1 wild type],

. . . . . . . . 130A [118A],

. . . . . . . . 183S [174S],

. . . . . . . . 188V [179V],

. . . . . . . . 195V [186V],

. . . . . . . . 198A [189A],

. . . . . . . . 211V [202V],

. . . . . . . . 217N [208N],

. . . . . . . . 231P [222P] [H7N9 Novel],

. . . . . . . . . . . . . . . . .[H7N9 LookAside_u],

. . . . . . . . . . . . . . . . .[pH1N1 LookAside_u],

. . . . . . . . . . . . . . . . .[H9N2 Hong Kong Avian Rare 2012],

. . . . . . . . . . . . . . . . .[H7N7 Avian Rare 1975-1976 ],

. . . . . . . . . . . . . . . . .[H6N1 Avian Singular 2002],

. . . . . . . . . . . . . . . . .[H6N2, N3, N5, N6, N7, N8],

. . . . . . . . 235L [226L],

. . . . . . . . 285N [277N],

. . . . . . . . 307D [299D],

. . . . . . . . 321R [313R],

. . . . . . . . 410N [401N],

. . . . . . . . 427I [418I],

. . . . . . . . 455D [446D],

. . . . . . . . 462K [453K],

. . . . . . . . syn517L (tTA) [syn508L (tTA)] [Emergent H7N9 Novel],

. . . . . . . . . . . . . . . . . . [H7N9 North America Avian wild type (tTg)],

. . . . . . . . . . . . . . . . . . [pH1N1 (tTg)],

. . . . . . . . . . . . . . . . . . [H1N1v Human 2012-2013 (tTg)

. . . . . . . . . . . . . . . . . . . . . . . . . including Arkansas14_2013_09_06],

. . . . . . . . . . . . . . . . . . [H1N1 Seasonal 1951],

. . . . . . . . . . . . . . . . . . [H5N1 Human and Avian 2013 (tTg)

. . . . . . . . . . . . . . . . . . . . . . . . . including Recent Fatality,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . IndonesiaNIHRD13157_f
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30mM 2013_06_18],

. . . . . . . . . . . . . . . . . . [H3N2],

. . . . . . . . . . . . . . . . . . [H7N7],

. . . . . . . . . . . . . . . . . . [H6N1 Rare Count < 10],

. . . . . . . . 541V [533V])

NA Polymorphisms

. . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/22/2013

. . . . . . . . Novel Neuraminidase, 1 Case Over 2 Sequences

. . . . . . . . Neuraminidase Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s

. . . . . . . . GISAID NA EPI477412

. . . . . . . . GISAID Isolate EPI_ISL_148728

. . . . . . . . 39 Polymorphisms (15 Amino and 24 Silent)

. . . . . . . . 16I [16I],

. . . . . . . . 19A [19A],

. . . . . . . . 40G [40G],

. . . . . . . . 53T [53T],

. . . . . . . . 81T [80T],

. . . . . . . . 84N [83N],

. . . . . . . . 112S [111S],

. . . . . . . . 241I [240I],

. . . . . . . . 266E [265E] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . 327T [325T] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [H9N2 wild type],

. . . . . . . . 335I [332I],

. . . . . . . . 346D [342D] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [pH1N1 First Base Donor GGA wild type],

. . . . . . . . 359A [355A],

. . . . . . . . 401A [397A],

. . . . . . . . 465R [464R] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [H9N2 Second Base Donor AGT])

. . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/DTID-ZJU10/2013

. . . . . . . . renamed after 2013-10-18 Deposit from

. . . . . . . . . . . . A/Zhejiang/DTID-ZJU05/2013

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU05_35M_2013_10_14_s

. . . . . . . . Novel Neuraminidase, 1 Case Over 2 Sequences

. . . . . . . . Neuraminidase Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s

. . . . . . . . GISAID NA EPI477451

. . . . . . . . GISAID Isolate EPI_ISL_148744

. . . . . . . . 39 Polymorphisms (15 Amino and 24 Silent)

. . . . . . . . 16I [16I],

. . . . . . . . 19A [19A],

. . . . . . . . 40G [40G],

. . . . . . . . 53T [53T],

. . . . . . . . 81T [80T],

. . . . . . . . 84N [83N],

. . . . . . . . 112S [111S],

. . . . . . . . 241I [240I],

. . . . . . . . 266E [265E] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . 327T [325T] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [H9N2 wild type],

. . . . . . . . 335I [332I],

. . . . . . . . 346D [342D] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [pH1N1 First Base Donor GGA wild type],

. . . . . . . . 359A [355A],

. . . . . . . . 401A [397A],

. . . . . . . . 465R [464R] [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . [H9N2 Second Base Donor AGT])

PB2 Polymorphisms

. . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/22/2013

. . . . . . . . GISAID PB2 EPI477407

. . . . . . . . GISAID Isolate EPI_ISL_148728

. . . . . . . . 18 Polymorphisms (3 Amino and 15 Silent)

. . . . . . . . syn58T (ACa),

. . . . . . . . syn149P (CCa),

. . . . . . . . syn153D (GAc),

. . . . . . . . syn160Q (CAa),

. . . . . . . . syn185I (ATa),

. . . . . . . . 197R [pH1N1],

. . . . . . . . syn288Q (CAa),

. . . . . . . . syn415R (AGg),

. . . . . . . . syn490S (AGt),

. . . . . . . . 559T,

. . . . . . . . 627E,

. . . . . . . . syn630R (AGa),

. . . . . . . . syn643S (TCa),

. . . . . . . . syn652N (AAc),

. . . . . . . . syn653S (TCc),

. . . . . . . . syn666T (ACa),

. . . . . . . . syn679P (CCa),

. . . . . . . . syn724V (GTa))

. . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/DTID-ZJU10/2013

. . . . . . . . renamed after 2013-10-18 Deposit from

. . . . . . . . . . . . A/Zhejiang/DTID-ZJU05/2013

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU05_35M_2013_10_14_s

. . . . . . . . Novel PolymeraseBasic2

. . . . . . . . Host Transition Potential

. . . . . . . . GISAID PB2 EPI477446

. . . . . . . . GISAID Isolate EPI_ISL_148744

. . . . . . . . 20 Polymorphisms (5 Amino and 15 Silent)

. . . . . . . . PB2 Truncated before aa5,

. . . . . . . . syn58T (ACa),

. . . . . . . . 60N [Host Transition Potential],
. . . . . . . . . . . [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . [pH1N1 2009-2011],

. . . . . . . . . . . [sH1N1 1981, 2006, 2008],

. . . . . . . . . . . [sH3N2 Hong Kong 1999],

. . . . . . . . . . . [zH3N2 North America 2011],

. . . . . . . . . . . [H5N1 Qinghai pika 2007],

. . . . . . . . . . . [H7N7 duck],

. . . . . . . . . . . [H9N2],

. . . . . . . . 87N [Host Transition Potential],
. . . . . . . . . . . [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . [pH1N1 2009 Widespread],

. . . . . . . . syn149P (CCa),

. . . . . . . . syn153D (GAc),

. . . . . . . . syn160Q (CAa),

. . . . . . . . syn185I (ATa),

. . . . . . . . 197R [pH1N1],

. . . . . . . . syn288Q (CAa),

. . . . . . . . syn415R (AGg),

. . . . . . . . syn490S (AGt),

. . . . . . . . 559T,

. . . . . . . . 627E,

. . . . . . . . syn630R (AGa),

. . . . . . . . syn643S (TCa),

. . . . . . . . syn652N (AAc),

. . . . . . . . syn653S (TCc),

. . . . . . . . syn666T (ACa),

. . . . . . . . syn679P (CCa),

. . . . . . . . syn724V (GTa))

PB1 Polymorphisms

. . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/22/2013

. . . . . . . . Novel PolymeraseBasic1

. . . . . . . . GISAID PB1 EPI477408

. . . . . . . . GISAID Isolate EPI_ISL_148728

. . . . . . . . 9 Polymorphisms (2 Amino and 7 Silent)

. . . . . . . . syn57T (ACa),

. . . . . . . . 195T [Host Transition Potential],
. . . . . . . . . . . . [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . [H5N1 Human Indonesia 2011],

. . . . . . . . . . . . [H5N1 Avian Asia 2002, 2003, 2004, 2007],

. . . . . . . . . . . . [pH1N1 Shenzhen 2009],

. . . . . . . . . . . . [sH1N1 Spain 2008],

. . . . . . . . . . . . [sH3N2 Boston 2011],

. . . . . . . . . . . . [H10N7 Avian 2007],

. . . . . . . . syn259A (GCa),

. . . . . . . . 361N [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . [H3N2 swine Thailand 2005],

. . . . . . . . . . . . [H9N2 gal Korea 2003],

. . . . . . . . . . . . [H1N3 anas Taiwan 2010],

. . . . . . . . syn365R (CGa),

. . . . . . . . syn463V (GTg),

. . . . . . . . syn544A (GCc),

. . . . . . . . syn630P (CCa),

. . . . . . . . syn659A (GCt))

. . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/DTID-ZJU10/2013

. . . . . . . . renamed after 2013-10-18 Deposit from

. . . . . . . . . . . . A/Zhejiang/DTID-ZJU05/2013

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU05_35M_2013_10_14_s

. . . . . . . . Novel PolymeraseBasic1

. . . . . . . . GISAID PB1 EPI477447

. . . . . . . . GISAID Isolate EPI_ISL_148744

. . . . . . . . 12 Polymorphisms (5 Amino and 7 Silent)

. . . . . . . . PB1 Truncated before aa31,

. . . . . . . . 31R,

. . . . . . . . 32D,

. . . . . . . . 45K,

. . . . . . . . syn57T (ACa),

. . . . . . . . 195T [Host Transition Potential],
. . . . . . . . . . . . [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . [H5N1 Human Indonesia 2011],

. . . . . . . . . . . . [H5N1 Avian Asia 2002, 2003, 2004, 2007],

. . . . . . . . . . . . [pH1N1 Shenzhen 2009],

. . . . . . . . . . . . [sH1N1 Spain 2008],

. . . . . . . . . . . . [sH3N2 Boston 2011],

. . . . . . . . . . . . [H10N7 Avian 2007],

. . . . . . . . syn259A (GCa),

. . . . . . . . 361N [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . [H3N2 swine Thailand 2005],

. . . . . . . . . . . . [H9N2 gal Korea 2003],

. . . . . . . . . . . . [H1N3 anas Taiwan 2010],

. . . . . . . . syn365R (CGa),

. . . . . . . . syn463V (GTg),

. . . . . . . . syn544A (GCc),

. . . . . . . . syn630P (CCa),

. . . . . . . . syn659A (GCt),

. . . . . . . . PB1 Truncated after aa755)

PA Polymorphisms

. . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/22/2013

. . . . . . . . Polymerase Acidic Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s

. . . . . . . . GISAID PA EPI477409

. . . . . . . . GISAID Isolate EPI_ISL_148728

. . . . . . . . 9 Polymorphisms (0 Amino and 9 Silent)

. . . . . . . . syn58G (GGc) [Emergent H7N9 wild type],

. . . . . . . . . . . . . . . . . .[H5N1 China, Xinjiang Human Fatality (wet market)],

. . . . . . . . . . . . . . . . . .[H5N1 Vietnam Human Fatality],

. . . . . . . . . . . . . . . . . .[H9N2 China, Hangzhou quail 2013-04-04],

. . . . . . . . syn109L (tTG) [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . . [H7N9 Avian wild type],

. . . . . . . . . . . . . . . . . . [H7N7 Asia Avian],

. . . . . . . . . . . . . . . . . . [H7N6 Japan quail 2009],

. . . . . . . . . . . . . . . . . . [H9N2 China, Guangdong environment 2013-05],

. . . . . . . . . . . . . . . . . . [H9N2 China, Zhejiang anas wild 2013-03-28],

. . . . . . . . . . . . . . . . . . [H5N1 China Human Fatality],

. . . . . . . . . . . . . . . . . . [H5N1 Asia Avian 2010-2012],

. . . . . . . . . . . . . . . . . . [H5N1 China Avian 2009],

. . . . . . . . . . . . . . . . . . [H5N2 England passerine 1991],

. . . . . . . . . . . . . . . . . . [H3N2 Vietnam swine 2010],

. . . . . . . . . . . . . . . . . . [H1N1 Human 1951],

. . . . . . . . . . . . . . . . . . [H1N1 US avian 2009, 2011],

. . . . . . . . . . . . . . . . . . [H1N1 US swine 2004,2005],

. . . . . . . . . . . . . . . . . . [H1N2 US swine 2012],

. . . . . . . . . . . . . . . . . . [H1N2 South Africa ostrich 2011],

. . . . . . . . syn147I (ATc),

. . . . . . . . syn163L (CTc),

. . . . . . . . syn236F (TTt),

. . . . . . . . syn502G (GGg),

. . . . . . . . syn608T (ACa),

. . . . . . . . syn625P (CCc),

. . . . . . . . syn673R (cGG))

. . . . ChinaZhejiangShaoxingDTIDZJU10_35M_2013_10_14_s (

. . . . . . . . A/Zhejiang/DTID-ZJU10/2013

. . . . . . . . renamed after 2013-10-18 Deposit from

. . . . . . . . . . . . A/Zhejiang/DTID-ZJU05/2013

. . . . . . . . . . . . ChinaZhejiangShaoxingDTIDZJU05_35M_2013_10_14_s

. . . . . . . . Polymerase Acidic Identical to

. . . . . . . . . . . . ChinaZhejiangShaoxing22_35M_2013_10_14_s

. . . . . . . . GISAID PA EPI477448

. . . . . . . . GISAID Isolate EPI_ISL_148744

. . . . . . . . 9 Polymorphisms (0 Amino and 9 Silent)

. . . . . . . . PA Truncated before aa8,

. . . . . . . . syn58G (GGc) [Emergent H7N9 wild type],

. . . . . . . . . . . . . . . . . .[H5N1 China, Xinjiang Human Fatality (wet market)],

. . . . . . . . . . . . . . . . . .[H5N1 Vietnam Human Fatality],

. . . . . . . . . . . . . . . . . .[H9N2 China, Hangzhou quail 2013-04-04],

. . . . . . . . syn109L (tTG) [Emergent H7N9 Novel:

. . . . . . . . . . . . . . . . . . . . . . 1 Case Over 2 Sequences],

. . . . . . . . . . . . . . . . . . [H7N9 Avian wild type],

. . . . . . . . . . . . . . . . . . [H7N7 Asia Avian],

. . . . . . . . . . . . . . . . . . [H7N6 Japan quail 2009],

. . . . . . . . . . . . . . . . . . [H9N2 China, Guangdong environment 2013-05],

. . . . . . . . . . . . . . . . . . [H9N2 China, Zhejiang anas wild 2013-03-28],

. . . . . . . . . . . . . . . . . . [H5N1 China Human Fatality],

. . . . . . . . . . . . . . . . . . [H5N1 Asia Avian 2010-2012],

. . . . . . . . . . . . . . . . . . [H5N1 China Avian 2009],

. . . . . . . . . . . . . . . . . . [H5N2 England passerine 1991],

. . . . . . . . . . . . . . . . . . [H3N2 Vietnam swine 2010],

. . . . . . . . . . . . . . . . . . [H1N1 Human 1951],

. . . . . . . . . . . . . . . . . . [H1N1 US avian 2009, 2011],

. . . . . . . . . . . . . . . . . . [H1N1 US swine 2004,2005],

. . . . . . . . . . . . . . . . . . [H1N2 US swine 2012],

. . . . . . . . . . . . . . . . . . [H1N2 South Africa ostrich 2011],

. . . . . . . . syn147I (ATc),

. . . . . . . . syn163L (CTc),

. . . . . . . . syn236F (TTt),

. . . . . . . . syn502G (GGg),

. . . . . . . . syn608T (ACa),

. . . . . . . . syn625P (CCc),

. . . . . . . . syn673R (cGG))

Please visit GeneWurx.com for insight into the latest published studies.

eH7N9 Influenza PolymeraseBasic2, PolymeraseBasic1, PolymeraseAcidic, Hemagglutinin & Neuraminidase Segments for
the Zhejiang CDC and Zhejiang University
elucidated respectively at
2013-10-18-13_58_23_165540 and 2013-10-18-16_02_21_195590 
by GeneWurx see.PolyDetector v0, Copyright 2007-2013.

Publication Copyright 2007-2013 GeneWurx.com, All Rights Reserved.

Human H7N9 Antigen & Polymerase Constellations, Guangdong v. Hong Kong

Sequences discussed in this analysis are variously stored publicly at GenBank and at GISAID. We gratefully acknowledge the authors, originating and submitting laboratories of the sequences from GenBank and from GISAID’s EpiFlu™ Database on which this research is based. A GISAID-generated list is detailed in a linked Excel for completeness in citation.

Publish Date : 2013-12-07
Last Update : 2013-12-09

Human H7N9 Segment Comparative
Emergent H7N9 GuangdongHuizhou1 51F 2013_08_10 Seg Origin Finding  HA   H7N9   Novel   NA   H7N9   Standard   PB2   H9N2   ReAssort   PB1   H9N2   ReAssort   PA   H7N9   Novel	Emergent H7N9 HongKong5942 36F 2013_11_30 Seg Origin Finding  HA   H7N9   Similar, but NOT Matched, to Guangdong   NA   H7N9   Novel   PB2   H9N2   ReAssort, Similar, but NOT Matched, to Guangdong   PB1   H9N2   ReAssort, NOT Similar to Guangdong   PA   H9N2   ReAssort
Deposit Report	Deposit Report

Please visit GeneWurx.com for insight into the latest published studies.

Publication Copyright 2007-2013 GeneWurx.com, All Rights Reserved.

Emergent H7N9 Vaccine Candidate Manifests Rare Pandemic H1N1 HA 131T

Sequences discussed in this analysis are variously stored publicly at GenBank and at GISAID. We gratefully acknowledge the authors, originating and submitting laboratories of the sequences from GenBank and from GISAID’s EpiFlu™ Database on which this research is based. A GISAID-generated list is detailed in a linked Excel for completeness in citation.

Publish Date : 2013-12-06
Last Update : 2013-12-06

On Wednesday, November 20, 2013, the National Institute of Infectious Diseases of Japan produced the details at GISAID of an Investigational Vaccine Candidate for Emergent H7N9 based on the Anhui1 base sequence. The Anhui Chuzhou patient is indicated as Case #3 on the 2013 H7N9 Human Case List maintained by the crowd-sourced FluTrackers.com. Evidently, when the NIID lab's candidate culturing process was modified with an initial Lewis Lung Carcinoma passage, a novel polymorphism to Emergent H7N9 manifested.

That H7N9 host switching revision, HA 131T, also features on a group of avian-centric human pH1N1 sequences we have been following that carries an unusually strong polymorphic homology to H7N9.  The HA 131T has previously been seen in H7N9 Avian, the H7N7 human fatality, current H3N2 Human Seasonal (wild type), H1N1 Seasonal 2008, H1N1 Variant Human and in 1918.  Two of the fatalities from the 2006 Egyptian H5N1 HA 230I-bearing Gharbiyah cluster carry this HA 131T.  Animal occurrences include H3N2, avH1N1, H5N1 (including a parrot) and H6N1.

We note when a Group 1 serotype (H1) develops contemporaneous homology with Group 2 serotypes (H3 & H7).  Evaluating the small, but persisting, Off-Clade pH1N1 set found primarily in western Africa alongside the dynamics of this Emergent H7N9 vaccine candidate may identify factors involved in minority strain maturation.

HA Polymorphisms

. . . . ChinaAnhuiChuzhou1_LLC1E3_35F_2013_03_15_f (

. . . . . . . . A/Anhui/1/2013 NIIDRG-10.1

. . . . . . . . Laboratory Derived Investigational Vaccine Candidate

. . . . . . . . Initial Passage in Lewis Lung Carcinoma culture

. . . . . . . . Clinical: 2013-03-09 Symptom Onset

. . . . . . . . Clinical: 2013-03-14 Fever 39° C, OutPatient AntiViral

. . . . . . . . Clinical: 2013-03-18 Fever 40° C, OutPatient AntiViral

. . . . . . . . Clinical: 2013-03-19 InPatient, Pneumonia

. . . . . . . . Clinical: 2013-03-20 Transfer Critical Care, Severe Pneumonia,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Septic Shock, Heart Inflammation,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Liver Dysfunction, Acute Kidney Damage,

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Brain Inflammation

. . . . . . . . Clinical: 2013-04-02 Remains in Critical Care

. . . . . . . . Clinical: 2013-04-09 Fatality

. . . . . . . . Clinical Tx: ECMO, Continuous Renal Replacement Therapy

. . . . . . . . Clinical Rx: Oseltamivir, Steroid, IV Immunoglobulin, Antibiotic Package

. . . . . . . . GISAID HA EPI486492

. . . . . . . . GISAID Isolate EPI_ISL_150198

. . . . . . . . 105 Polymorphisms (18 Amino and 87 Silent)

. . . . . . . . 11I [#4I],

. . . . . . . . 130A [118A],

. . . . . . . . 143T [131T],

. . . . . . . . 183S [174S],

. . . . . . . . 188V [179V],

. . . . . . . . 195V [186V],

. . . . . . . . 198A [189A],

. . . . . . . . 211V [202V],

. . . . . . . . 217N [208N],

. . . . . . . . 235L [226L],

. . . . . . . . 285N [277N],

. . . . . . . . 307D [299D],

. . . . . . . . 321R [313R],

. . . . . . . . 410N [401N],

. . . . . . . . 427I [418I],

. . . . . . . . 455D [446D],

. . . . . . . . 462K [453K],

. . . . . . . . 541V [533V])

NA Polymorphisms

. . . . ChinaAnhuiChuzhou1_LLC1E3_35F_2013_03_15_f (

. . . . . . . . A/Anhui/1/2013 NIIDRG-10.1

. . . . . . . . Laboratory Derived

. . . . . . . . GISAID NA EPI486493

. . . . . . . . GISAID Isolate EPI_ISL_150198

. . . . . . . . 33 Polymorphisms (10 Amino and 23 Silent)

. . . . . . . . 16I [16I],

. . . . . . . . 19A [19A],

. . . . . . . . 40G [40G],

. . . . . . . . 53T [53T],

. . . . . . . . 81T [80T],

. . . . . . . . 84N [83N],

. . . . . . . . 112S [111S],

. . . . . . . . 335I [332I],

. . . . . . . . 359A [355A],

. . . . . . . . 401A [397A])

Please visit GeneWurx.com for insight into the latest published studies.

eH7N9 Influenza Hemagglutinin & Neuraminidase Segments
elucidated at 2013-12-06-03_37_46_264340
by GeneWurx see.PolyDetector v0, Copyright 2007-2013.

Publication Copyright 2007-2013 GeneWurx.com, All Rights Reserved.

First Hong Kong Human Case, 36F, Repeats H3N2 Antigenic HA 128A

Sequences discussed in this analysis are variously stored publicly at GenBank and at GISAID. We gratefully acknowledge the authors, originating and submitting laboratories of the sequences from GenBank and from GISAID’s EpiFlu™ Database on which this research is based. A GISAID-generated list is detailed in a linked Excel for completeness in citation.

Publish Date : 2013-12-05
Last Update : 2014-04-07

On 2013-12-04, the Centre for Health Protection of Hong Kong released a full set of sequences at GISAID from the most recent Emergent H7N9 human case.  The 36 year old female domestique was sampled in Hong Kong on 2013-11-30 during hospitalisation.  She was variously reported to be from Indonesia and India, although the Indonesian consulate has confirmed her nationality at this time.  Emergent H7N9 went undetected on the first two Influenza tests

Four home contacts and a patient who shared a ward at Tuen Mun Hospital have exhibited symptoms.  Twelve Health Care Workers who attended the patient have also exhibited symptoms.

The Emergent H7N9 human reservoir once again displays fecundity with yet another novel constellation of ReAssortment gaining internal genes from the H9N2 reservoir, including all three of the Polymerase Complex (Segments 1,2,3). Within this Polymerase Complex, only segment 1, PB2, resembles the previous human ReAssortment from Guangdong, notably at a distance of 8 residues.  Hypermorphic behaviour is certainly now in evidence from this host-transition reservoir.

HA 128A repeats on this second reported Emergent H7N9 sequence with an epidemiological relationship to Guangdong province, i.e. GuangdongHuizhou1_51F_2013_08_10_s.  This HA 128A antigenic variation is currently circulating world-wide from the Season 2012 Human H3N2 reservoir (Record-Setting Fatalities and Hospitalisations).  HA 128A features on the Russian sequence from the recent GeneWurx H3N2 dual geography Prediction Validation report concerning another potential antigenic site revision.

A comprehensive collection of official reports and journalists' filings covering the events surrounding this latest human Emergent H7N9 case may be accessed at the crowd-sourced, FluTrackers forum [214444] thread.  Hong Kong Department of Health reports are in evidence, including descriptions of multiple diagnostic testing failures while influenza symptoms were present in this patient, in the patient's employers and in hospital contacts of the patient.  This Hong Kong index patient is indicated as Case #142 on the 2013 H7N9 Human Case List maintained by the FT moderator and forum members. Due to testing irregularities, no other cases are presently H7N9-confirmed in these multiple symptomatic clusters having a shared focus.

Activity tracing relates recent travel by the patient to the mainland, specifically Shenzhen, Guangdong, on November 17^th.  Bloomberg reports that Hong Kong residents log more than 30 million trips each year to the popular Shenzhen day-trip destination. Dr. Ian Mackay (University of Queensland), H7N9 Visualiser, adroitly describes viral spread proclivity in the Bloomberg article and at his publication center, Virology Down Under, Editor's Note #12:

“Respiratory viruses do their own thing; they don’t respect boundaries,” said Ian Mackay, an associate professor of clinical virology at the University of Queensland in Brisbane, Australia, in a telephone interview. “It does seem that it’s continuing to add to provinces and regions, rather than reappear in all the old places it started in back in February and March.”

Although the first Hong Kong human case sequence does resemble the first Guangdong human case sequence on the HA and loosely on a similar, but certainly not matching, H9N2-like PB2, HongKong5942_M_36F_2013_11_30_s manifests a distinct segment constellation with variant H9N2-like PA and PB1 segments.  A novel polymorphism to Emergent H7N9 also arises on the Neuraminidase, NA 264V.  That NA revision has been found co-located on pH1N1 sequences with HA 230I.

With HA 128A, an H3N2 human seasonal circulation, appearing to fix over the course of three months and the novelty of NA 264V, a human  pH1N1 revision, the Hong Kong case gains human-hosted genetics and potentially functional infectivity.  The fresh Polymerase complex is suggestive that reassortment comes with ease for these current H7N9 genetics.  After noting that H5N1 acquired a full Polymerase complex from Emergent H7N9 during early April 2013 in presumable Wet Market Surveillance at Hangzhou, Zhejiang Province, we began to understand the attractant allegiance these cross-group reservoirs share.

What GeneWurx had originally marked as mere fealty is now documented as practical sycophantic bi-directionality.

At any estimation, diagnostic testing was eluded successfully in many cases on this particular background.  Potential involvement of two circulating human disease reservoirs as donors (pH1N1 & sH3N2) increases the risk of silent spread leading to wider human morbidity.

HA Polymorphisms

. . . . HongKong5942_M_36F_2013_11_30_s (

. . . . . . . . A/Hong Kong/5942/2013

. . . . . . . . Clinical: 2013-11-21 Cough
. . . . . . . . Clinical: 2013-11-25 Sought Medical Assistance, Private Doctor
. . . . . . . . Clinical: 2013-11-27 Shortness of Breath, Tuen Mun Hospital Admission
. . . . . . . . Clinical: 2013-11-29 Tuen Mun Hospital Critical Care
. . . . . . . . Clinical: 2013-11-30 Queen Mary Hospital Transfer
. . . . . . . . Clinical: 2013-12-07 ECMO complete, Oxygen Continues
. . . . . . . . Clinical: 2013-12-07 Oxygen continues
. . . . . . . . Clinical: 2013-12-07 Remains in Critical Care

. . . . . . . . Clinical Tx: ECMO

. . . . . . . . GISAID HA EPI490882

. . . . . . . . GISAID Isolate EPI_ISL_151417

. . . . . . . . 104 Polymorphisms (19 Amino and 85 Silent)

. . . . . . . . 11I [#4I] [zH3N2],

. . . . . . . . 130A [118A],

. . . . . . . . 140A [128A] [H3N2 Human Seasonal],

. . . . . . . . 183S [174S] [zH3N2],

. . . . . . . . 188V [179V] [zH3N2],

. . . . . . . . 195V [186V],

. . . . . . . . 198A [189A],

. . . . . . . . 211V [202V] [zH3N2],

. . . . . . . . 217N [208N],

. . . . . . . . 235L [226L] [zH3N2],

. . . . . . . . 285N [277N] [zH3N2],

. . . . . . . . 300S [292S],

. . . . . . . . 307D [299D],

. . . . . . . . 321R [313R] [H9N2],

. . . . . . . . 410N [401N],

. . . . . . . . 427I [418I],

. . . . . . . . 455D [446D],

. . . . . . . . 462K [453K] [zH3N2],

. . . . . . . . 541V [533V])

NA Polymorphisms

. . . . HongKong5942_M_36F_2013_11_30_s (

. . . . . . . . A/Hong Kong/5942/2013
. . . . . . . . Novel Neuraminidase

. . . . . . . . GISAID NA EPI490881

. . . . . . . . GISAID Isolate EPI_ISL_151417

. . . . . . . . 33 Polymorphisms (11 Amino and 22 Silent)

. . . . . . . . NA Truncated before aa10 [NA Truncated before aa10],

. . . . . . . . 16I [16I],

. . . . . . . . 19A [19A],

. . . . . . . . 40G [40G],

. . . . . . . . 53T [53T],

. . . . . . . . 81T [80T],

. . . . . . . . 84N [83N],

. . . . . . . . 112S [111S],

. . . . . . . . 264V [263V] [Emergent H7N9 Novel],
. . . . . . . . . . . . . . . . . [pH1N1 with HA 230I],
. . . . . . . . . . . . . . . . . [avH1N1_farm],
. . . . . . . . . . . . . . . . . [H5N1],
. . . . . . . . . . . . . . . . . [H6N1],

. . . . . . . . 335I [332I],

. . . . . . . . 359A [355A],

. . . . . . . . 401A [397A])

PB2 Polymorphisms

. . . . HongKong5942_M_36F_2013_11_30_s (

. . . . . . . . A/Hong Kong/5942/2013

. . . . . . . . ~ H9N2
. . . . . . . . Distant to Relative: Count 08 H7N9 Guangdong
. . . . . . . . Distant to Relative: Count 78 H7N9 Secondary
. . . . . . . . GISAID PB2 EPI490879

. . . . . . . . GISAID Isolate EPI_ISL_151417

. . . . . . . . 91 Polymorphisms (9 Amino and 82 Silent)

. . . . . . . . 139I [pH1N1 Rare: Early Emergence],

. . . . . . . . 191E [pH1N1 Wild Type],

. . . . . . . . 283I,

. . . . . . . . 286G,

. . . . . . . . 292I,

. . . . . . . . 559T,

. . . . . . . . 569A,

. . . . . . . . 570I,

. . . . . . . . 676V)

PB1 Polymorphisms

. . . . HongKong5942_M_36F_2013_11_30_s (

. . . . . . . . A/Hong Kong/5942/2013

. . . . . . . . ~ H9N2
. . . . . . . . Distant to Relative: Count 44 H5N1
. . . . . . . . Distant to Relative: Count 46 H7N9
. . . . . . . . Distant to Relative: Count 53 H7N9 Guangdong
. . . . . . . . GISAID PB1 EPI490880

. . . . . . . . GISAID Isolate EPI_ISL_151417

. . . . . . . . 46 Polymorphisms (4 Amino and 42 Silent)

. . . . . . . . 105H,

. . . . . . . . 178D,

. . . . . . . . 525I,

. . . . . . . . 682V)

PA Polymorphisms

. . . . HongKong5942_M_36F_2013_11_30_s (

. . . . . . . . A/Hong Kong/5942/2013

. . . . . . . . ~ H9N2
. . . . . . . . Distant to Relative: Count 58 H7N9
. . . . . . . . Distant to Relative: Count 74 H7N9 Guangdong
. . . . . . . . GISAID PA EPI490878

. . . . . . . . GISAID Isolate EPI_ISL_151417

. . . . . . . . 73 Polymorphisms (4 Amino and 69 Silent)

. . . . . . . . 100V,

. . . . . . . . 336M,

. . . . . . . . 356K [H5N1 Asia Human Fatalities], 

. . . . . . . . 394D)

Please visit GeneWurx.com for insight into the latest published studies.

eH7N9 Influenza PolymeraseBasic2, PolymeraseBasic1, PolymeraseAcidic, Hemagglutinin & Neuraminidase Segments
elucidated at 2013-12-05-02_53_59_107890 by GeneWurx see.PolyDetector v0, Copyright 2007-2013.

Publication Copyright 2007-2013 GeneWurx.com, All Rights Reserved.